workshops

Workshop 2.  IPS

Day 1:

Lectures:

  1. Basic Stem Cell Biology
  2. Pleuripotency: pathways and detection

Practical:

            Fibroblast culture:

  1. Isolation of fibroblasts from skin samples
  2. Culture and propagation of fibroblasts
  3. Characterization of fibroblasts in culture

Day 2: 

Lectures:

  1. IPS:  theory and applications
  2. Methods of induction of IPS

Practical:

  1. Transfection of cells for pleuripotency
  2. Culture of transfected cells

Day 3:

Lectures:

  1. Methods of selection of transfected cells
  2. Propagation of pleuripotent cells

Practical:

  1. Kill curve plotting
  2. Culture of viable cells
  3. RNA extraction from selected cells


 

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