Workshop 2.  IPS

Day 1:

Lectures:

1. Basic Stem Cell Biology
2. Pleuripotency: pathways and detection

Practical:

            Fibroblast culture:

1. Isolation of fibroblasts from skin samples
2. Culture and propagation of fibroblasts
3. Characterization of fibroblasts in culture

Day 2: 

Lectures:

1. IPS:  theory and applications
2. Methods of induction of IPS

Practical:

1. Transfection of cells for pleuripotency
2. Culture of transfected cells

Day 3:

Lectures:

1. Methods of selection of transfected cells
2. Propagation of pleuripotent cells

Practical:

1. Kill curve plotting
2. Culture of viable cells
3. RNA extraction from selected cells