workshops

Workshop 4:  Standardization of HSCs

Day 1:

Lectures:

  1. Types and Sources of HSCs
  2. Identification of HSCs
  3. Methods of viability detection

Practical:

  1. Mononuclear Cell Separation
  2. MSCs isolation
  3. Stem Cell count and viablity

Day 2:

Lectures:

  1. Significance of Flowcytometry in stem cell research
  2. Flowcytometry:  basic principles

Practical:

  1. Gating for stem cell experiments
  2. Surface Monoclonal antibody detection using   flowcytometry

Day 3:

Lectures:

  1. Surface and Intracellular antigen detection using       flowcytometry
  2. Cell sorting theories and methods

Practical:

  1. Immunomagnetic cell sorting
  2. Stem Cell count using flowcytometry (ISCHage protocol)

Related Articles

Leave a Reply

Check Also

Close
Close