workshops

Workshop 1:  Differentiation of MSCs

Day 1:

Lectures: 

  1. Origin and Isolation of MSCs
  2. Characteristics of MSCs

 

Practical Session:            

  1. Verification of MSCs before differentiation:
  2. Morphology
  3. Count and Viability

 

Day 2:

Lectures:

  1. Stem Cell Transdifferentiation:  theories and practice
  2. Trilineage differentiation of MSCs

Practical Sessions:

 

  1. Osteogenic differentiation
  2. Chondrogenic differentiation
  3. Adipognic Differentiation

Day 3:

Lectures:

  1. Evaluation of Stem Cell Plasticity
  2. Evaluation of MSC trilineage differentiation

Practical:

  1. Immunohistochemistry for osteogenic differentiation
  2. Immunohistochemistry for chrondrogenic differentiation
  3. Immunohistochemistry for adipogenic differentiation

 

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